Tomography and Cryo-FIB Workshop 2024

Midwest Center for Cryo-Electron Tomography

September 23 - 27, 2024

Image courtesy of Dr. Jae Yang, PhD

This workshop will help you learn the fundamentals of cryo-electron tomography (cryo-ET) including sample preparation, FIB-milling, correlative imaging, and cryo-ET data collection and analysis. Whether you are new to the field, have been collecting single-particle cryo-EM data and are now looking to start your first tomography project, or have experience with tomography, you'll come away with fresh ideas and strategies you can put to use right away. The morning sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only. Connect face-to-face with colleagues and build community with other tomography researchers at the Midwest Center for Cryo-Electron Tomography (MCCET) at the University of Wisconsin - Madison.

Topics include:

Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
Training in data acquisition workflows that include screening, data collection, and flexible scripting.
Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.
Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.
Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.

WHEN: Monday, September 23, 2024, to Friday, September 27, 2024

Breakfast begins at 8:00 a.m. followed by the morning program from 9 a.m. to 12:00 p.m.

Afternoon breakout sessions for hands-on use of instrumentation until 5:00 p.m.

Meals are included.

WHERE: HF DeLuca Biochemistry Building, University of Wisconsin, Madison

Note: In-person registration is now closed. Registration for virtual sessions is still open.

Morning sessions will be recorded for future viewing.

Additional information and schedule.

First Name*

Last Name*

Research Group | PI Name*

Academic registrants please list your lab group (First and Last name for PI)

What is your position or career level?*

Please describe if you are a Graduate Student, Postdoc, Principal Investigator, or Other

Email Address*

Institution or Company*

State or Province*

Country*

ERA Commons or ORCID (if available)

About yourself and your cryo-ET experience

Please note that only virtual session registrations are being accepted.

Virtual

Please describe your tomography experience*

Please describe your tomography data collection experience.*

Please describe your tomography data processing experience.*

Please describe your cryo-CLEM and cryo-FIB experience*

How have you previously applied cryo-EM or cryo-ET in your research?*

How will participating in this workshop help your research?*